Download Transgenesis Techniques PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9781592591787
Pages : 346 pages
Rating : 4.5/5 (592 users)

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Download Transgenesis Techniques PDF by Alan R. Clarke Full Free and published by Springer Science & Business Media. This book was released on 2002 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has witnessed a spectacular explosion in both the development and the use of transgenic technologies. In Transgenesis Techniques: Principles and Protocols, Second Edition, authoritative researchers-including members of the Dolly team-take stock of these exciting developments to present a collection of readily reproducible techniques for the generation and analysis of transgenic animals, focusing on the manipulation of the mammalian genome. This second edition devotes much space to the creation of genetically modified murine strains, providing access to the germline both by conventional pronuclear injection and by retroviral and adenoviral infection. Extensive coverage is also given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for both constitutive and conditional (Cre-lox or the Flp-frt systems) gene targeting. Additional chapters provide cutting-edge techniques for the cryopreservation of both male and female germlines, for the generation of transgenic sheep by nuclear transfer, and for the basic analysis of transgenic organisms. Comprehensive and highly practical, this second edition of Transgenesis Techniques: Principles and Protocols updates and expands the acclaimed first edition with a wealth of easy-to-follow cutting-edge protocols and practical tips for producing transgenic animals and analyzing their integrated transgenes.


Download Animal Transgenesis and Cloning PDF

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Publisher : Wiley-Blackwell
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ISBN 10 : STANFORD:36105111818394
Pages : 220 pages
Rating : 4.F/5 ( users)

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Download Animal Transgenesis and Cloning PDF by Louis-Marie Houdebine Full Free and published by Wiley-Blackwell. This book was released on 2003-04-02 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Designed to be a concise, balanced introduction to this dynamic subject, covering key issues and current techniques currently used in animal transgenesis and cloning. Provides the essentials of the subject, from the molecular basis of gene structure and function to therapeutic gene cloning in humans. Social and ethical implications of this important area of research are also considered.


Download Signaling in the Heart PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9781441994615
Pages : 507 pages
Rating : 4.4/5 (441 users)

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Download Signaling in the Heart PDF by José Marín-García Full Free and published by Springer Science & Business Media. This book was released on 2011-06-21 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: Signal transduction pathways are at the core of most biological processes and are critical regulators of heart physiology and pathophysiology. The heart is both a transmitter and dynamic receptor of a variety of intracellular and extracellular stimuli, playing a critical role of an integrator of diverse signaling mechanisms. Alterations in signaling pathways are contributing factors in the development and progression of a broad spectrum of diseases, ranging from dysrhythmias and atherosclerosis to hypertension and the metabolic syndrome. Targeting specific components of these signaling pathways has been shown to be effective in preclinical studies with significant therapeutic impact. This book brings together current knowledge in cardiovascular cell signal transduction mechanisms, advances in novel therapeutic approaches to improve cardiac function, and discussion of future directions. Presented from a post-genomic perspective, this exciting book introduces important new ideas in cardiovascular systems biology. It is an invaluable reference for cardiology researchers and practitioners.


Download Transgenic Animal Technology PDF

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Publisher : Gulf Professional Publishing
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ISBN 10 : 9780125571661
Pages : 635 pages
Rating : 4.1/5 (125 users)

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Download Transgenic Animal Technology PDF by Carl A. Pinkert Full Free and published by Gulf Professional Publishing. This book was released on 2002-11-18 with total page 635 pages. Available in PDF, EPUB and Kindle. Book excerpt: A transgenic animal is one that is genetically modified to carry genes from another species. Transgenic species can be raised to carry potentially useful genes from a variety of species. While the topics of genetic engineering and cloning are controversial, the reality is that these technologies offer tremendous benefits to society - from offering a framework for developing and screening medical therapies, to enhancing the safety and nutrition of the food we eat. One potential application of research into transgenic animal technology is the creation of domestic animals genetically designed to express a certain human disease and therefore serve as models for the study and treatment of human illnesses. Although many mouse models of human diseases are available today, such models in large domestic animals physiologically more similar to humans are sparse and critically needed. Further research in this field will undoubtedly uncover many more direct and indirect benefits of this technology. Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols New section on analysis


Download Principles of Development PDF

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Publisher : Oxford University Press, USA
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ISBN 10 : 9780198709886
Pages : 721 pages
Rating : 4.1/5 (198 users)

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Download Principles of Development PDF by Lewis Wolpert Full Free and published by Oxford University Press, USA. This book was released on 2015 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the key principles of developmental biology that students need to know, which are underpinned throughout by experimental evidence, and an exploration of the molecular basis of the subject.


Download An Introduction to Molecular Biotechnology PDF

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Publisher : John Wiley & Sons
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ISBN 10 : 9783527812868
Pages : 544 pages
Rating : 4.5/5 (527 users)

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Download An Introduction to Molecular Biotechnology PDF by Michael Wink Full Free and published by John Wiley & Sons. This book was released on 2020-12-03 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Completely updated in line with the rapid progress made in the field, this new edition of the highly-praised textbook addresses powerful new methods and concepts in biotechnology, such as genome editing, reprogrammed stem cells, and personalized medicine. An introduction to the fundamentals in molecular and cell biology is followed by a description of standard techniques, including purification and analysis of biomolecules, cloning techniques, gene expression systems, genome editing methods, labeling of proteins and in situ-techniques, standard and high resolution microscopy. The third part focuses on key areas in research and application, ranging from functional genomics, proteomics and bioinformatics to drug targeting, recombinant antibodies and systems biology. The final part looks at the biotechnology industry, explaining intellectual property issues, legal frameworks for pharmaceutical products and the interplay between start-up and larger companies. The contents are beautifully illustrated throughout, with hundreds of full color diagrams and photographs. Provides students and professionals in life sciences, pharmacy and biochemistry with everything they need to know about molecular biotechnology.


Download Animal Biotechnology PDF

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Publisher : Firewall Media
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ISBN 10 : 813180108X
Pages : 188 pages
Rating : 4.8/5 (81 users)

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Download Animal Biotechnology PDF by Manjula Shenoy Full Free and published by Firewall Media. This book was released on 2007 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Download Transgenic Ascidians PDF

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Publisher : Springer
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ISBN 10 : 9789811075452
Pages : 201 pages
Rating : 4.8/5 (811 users)

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Download Transgenic Ascidians PDF by Yasunori Sasakura Full Free and published by Springer. This book was released on 2018-03-14 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies. Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates. This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.


Download Marine Genetics PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9789401721844
Pages : 219 pages
Rating : 4.4/5 (41 users)

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Download Marine Genetics PDF by Antonio M. Solé-Cava Full Free and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our current knowledge of marine organisms and the factors affecting their ecology, distribution and evolution has been revolutionised by the use, in the last 20 years, of molecular population genetics tools. This book is the result of a meeting of world-leading experts, in Rio de Janeiro, where the state of the art of this field was reviewed. Topics covered include the molecular analysis of bio-invasions, the recent developments in marine biotechnology, the factors affecting levels of genetic variation and population structure in marine organisms and their application to conservation biology, fisheries and aquaculture. This is the first book dedicated to the genetic study of marine organisms. It will be very useful to biology students, scientists and anyone working or simply interested in areas such as marine biology, zoology, ecology, and population and molecular genetics.


Download Transgenic Animals PDF

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Publisher : Tolley
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ISBN 10 : UOM:39015018867492
Pages : 358 pages
Rating : 4.3/5 (39 users)

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Download Transgenic Animals PDF by Symposium on Transgenetic Technology in Medicine and Agriculture (1988, Bethesda, Md.) Full Free and published by Tolley. This book was released on 1991 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transgenesis involves injecting foreign genetic material into an animal's own genetic material to produce specific, economically significant traits. This book details the techniques necessary for the production of transgenic animals and the industrial application of these techniques.


Download TRANSGENIC ANIMAL PATENT REFORM ACT OF 1989 PDF

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Publisher :
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ISBN 10 : UCR:31210014712499
Pages : 696 pages
Rating : 4.3/5 (312 users)

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Download TRANSGENIC ANIMAL PATENT REFORM ACT OF 1989 PDF by United States. Congress. House. Committee on the Judiciary Full Free and published by . This book was released on 1988 with total page 696 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Download Dietary Supplements for the Health and Quality of Cultured Fish PDF

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Publisher : CABI
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ISBN 10 : 9781845931995
Pages : 256 pages
Rating : 4.8/5 (845 users)

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Download Dietary Supplements for the Health and Quality of Cultured Fish PDF by Heisuke Nakagawa Full Free and published by CABI. This book was released on 2007-01-01 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proper nutrition and feeding are critical components of aquaculture. Effective utilization of well-balanced diet formulations is of utmost importance and influences most aspects of production, including the cost, yield and quality of resulting products, as well as the sustainability of aquacultural practices including potential environmental impacts. In addition to the various required macro- and micro-nutrients, emphasis has been placed on non-nutritive supplements in recent years. These have been found to influence many attributes of freshwater and marine fish, particularly taste and colour, resistance to disease and health benefits to human consumers. This book presents a comprehensive review of the most important nutrients and non-nutritive supplements, using scientific evidence to support the best practices for using these compounds in diet formulations to enhance the efficiency and sustainability of aquaculture. This book is a valuable resource for students and practitioners in aquaculture and fisheries, as well as animal nutrition, feeding and husbandry.


Download Transgenic Plants PDF

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Publisher : BoD – Books on Demand
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ISBN 10 : 9789535101819
Pages : 494 pages
Rating : 4.5/5 (535 users)

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Download Transgenic Plants PDF by Yelda Ozden Çiftçi Full Free and published by BoD – Books on Demand. This book was released on 2012-03-07 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: Development of efficient transformation protocols is becoming a complementary strategy to conventional breeding techniques for the improvement of crops. Thus, Transgenic Plants - Advances and Limitations covers the recent advances carried on improvement of transformation methods together with assessment of the impact of genetically transformed crops on biosafety. Each chapter has been written by one or more experienced researchers in the field and then carefully edited to ensure throughness and consistency.


Download Transgenic Plants PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9781592598274
Pages : 437 pages
Rating : 4.5/5 (592 users)

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Download Transgenic Plants PDF by Leandro Peña Full Free and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.


Download Transgenesis PDF

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Publisher : Humana
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ISBN 10 : 1071629891
Pages : 0 pages
Rating : 4.6/5 (629 users)

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Download Transgenesis PDF by Thomas L. Saunders Full Free and published by Humana. This book was released on 2023-04-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.


Download Clinical Aspects of Hearing PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 0387978429
Pages : 338 pages
Rating : 4.9/5 (978 users)

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Download Clinical Aspects of Hearing PDF by Thomas R. VanDeWater Full Free and published by Springer Science & Business Media. This book was released on 1996 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last two decades of the twentieth century have witnessed advances in our knowledge of the biological processes which result in hearing. These have come about from the application of contemporary molecular biological, physiology, and imaging techniques in combination with discoveries in the domain of hearing. This volume presents these pivotal developments and their applications to the understanding of normal and abnormal hearing in humans, information that is essential to the ability of both scientists and clinicians to further their understanding and treatment of hearing disorders.


Download Insect Transgenesis PDF

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Publisher : CRC Press
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ISBN 10 : 0367398389
Pages : 424 pages
Rating : 4.3/5 (398 users)

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Download Insect Transgenesis PDF by Taylor & Francis Group Full Free and published by CRC Press. This book was released on 2020-12-18 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Imagine scientists controlling the transmission of certain diseases through the genetic modification of mosquitoes. Eradicating harmful insects without the use of pesticides. Or increasing the fertility of some insects who in turn eat harmful arthropods or even a plant pathogen. Those are just a few of the real-world applications of insect transgenesis, which offers substantial benefits to humankind-whether it be in improving agricultural productivity or reducing the spread of insect-vectored diseases. Insect Transgenesis: Methods and Applications is the first publication to describe in a comprehensive manner the various methodologies available, possible applications, and the risk assessment and regulatory issues involved in this fascinating area of research. Divided into several areas of interest, the book starts with an overview of the history and methodology of insect gene transfer. The book then examines gene targeting by homologous recombination and recombination systems, and systems for transgenic selection, including visible eye color markers, chemical resistance, and fluorescent proteins. Other sections consider the use of various vector systems to integrate DNA into a host genome or to express foreign genes in a host organism. The work concludes with strategies for the use of transgenic insects, including examples for agricultural pests and vectors of disease. Of particular interest are the final chapters that discuss risk assessment considerations and governmental regulatory procedures for the transport and release of transgenic insects.