Download Monoclonal Antibodies and Functional Cell Lines PDF

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Publisher : Springer
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ISBN 10 : 1468446754
Pages : 426 pages
Rating : 4.4/5 (446 users)

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Download Monoclonal Antibodies and Functional Cell Lines PDF by Roger H. Kennett Full Free and published by Springer. This book was released on 2012-12-12 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume serves as a follow-up to our previous book, MonoclonalAntibodies Hybridomas: A New Dimension in Biological Analyses. We continue the theme of monoclonal antibodies and their applications, attempting to cover some of the areas not covered in the previous volume. We again include an appendix de scribing methods useful to those who ar-e beginning to apply these techniques in their own laboratories. This volume will be followed by another concentrating on the combination of monoclonal antibody techniques with molecular genetic techniques to study structure/function relationships at the level of both the gene and gene product. Roger H. Kennett Kathleen B. Bechtol Philadelphia, Pennsylvania Thomas J. McKearn Princeton, New Jersey IX Acknowledgments Roger Kennett acknowledges the patience and support of his wife, Carol, and his family, friends, and colleagues during the work on this volume, and again thanks, above all, the Lord, Jesus Christ. Kathleen Bechtol wishes to thank colleagues and friends for their support and understanding during the months of preparation of this volume. Tom McKearn acknowledges and thanks his wife, Pat, and his family for their support and encouragement. Xl Contents PART I INTRODUCTION 1 Introduction: Reflections on Nine Years of Monoclonal Antibodies from Hybridomas 3 ROGER H. KENNETT, KATHLEEN B. BECHTOL, AND THOMAS J. McKEARN 1. Biotechnology'S "Coming of Age". . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II. Monoclonal Antibodies-An Overview of Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 III. Commercialization of Monoclonal Antibody Technology.. . . .. ... . .... .... .. . ... . . 10 References. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 . . . . . . . . . . . . . . . . . . . . .


Download Monoclonal Antibodies and Functional Cell Lines PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9781468446739
Pages : 426 pages
Rating : 4.4/5 (468 users)

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Download Monoclonal Antibodies and Functional Cell Lines PDF by Roger H. Kennett Full Free and published by Springer Science & Business Media. This book was released on 2013-03-08 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume serves as a follow-up to our previous book, MonoclonalAntibodies Hybridomas: A New Dimension in Biological Analyses. We continue the theme of monoclonal antibodies and their applications, attempting to cover some of the areas not covered in the previous volume. We again include an appendix de scribing methods useful to those who ar-e beginning to apply these techniques in their own laboratories. This volume will be followed by another concentrating on the combination of monoclonal antibody techniques with molecular genetic techniques to study structure/function relationships at the level of both the gene and gene product. Roger H. Kennett Kathleen B. Bechtol Philadelphia, Pennsylvania Thomas J. McKearn Princeton, New Jersey IX Acknowledgments Roger Kennett acknowledges the patience and support of his wife, Carol, and his family, friends, and colleagues during the work on this volume, and again thanks, above all, the Lord, Jesus Christ. Kathleen Bechtol wishes to thank colleagues and friends for their support and understanding during the months of preparation of this volume. Tom McKearn acknowledges and thanks his wife, Pat, and his family for their support and encouragement. Xl Contents PART I INTRODUCTION 1 Introduction: Reflections on Nine Years of Monoclonal Antibodies from Hybridomas 3 ROGER H. KENNETT, KATHLEEN B. BECHTOL, AND THOMAS J. McKEARN 1. Biotechnology'S "Coming of Age". . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 II. Monoclonal Antibodies-An Overview of Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 III. Commercialization of Monoclonal Antibody Technology.. . . .. ... . .... .... .. . ... . . 10 References. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 . . . . . . . . . . . . . . . . . . . . .


Download Monoclonal Antibodies PDF

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Publisher : Cambridge University Press
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ISBN 10 : 0521425034
Pages : 500 pages
Rating : 4.4/5 (425 users)

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Download Monoclonal Antibodies PDF by Mary A. Ritter Full Free and published by Cambridge University Press. This book was released on 1995-01-26 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aims to provide a unique combination of the production (by both cellular and molecular biology techniques), structure and functional characteristics of monoclonal antibodies, together with detailed discussions of the various analytic, diagnostic and therapeutic applications.


Download Monoclonal Antibodies PDF

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Publisher : CRC Press
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ISBN 10 : 9781482242683
Pages : 232 pages
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Download Monoclonal Antibodies PDF by Heddy Zola Full Free and published by CRC Press. This book was released on 2013-11-20 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes, in detail, tested techniques for the produc-tion and use of monoclonal antibodies. It covers those aspects of interest to all scientists working with monoclonal antibodies and presents methods in a step-by-step format for easy refer-ence. The text serves as a laboratory manual; and discusses rationale behind each method, and th


Download Monoclonal Antibodies & T Cell Products PDF

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Publisher : CRC Press
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ISBN 10 : 9781000005943
Pages : 187 pages
Rating : 4.0/5 ( users)

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Download Monoclonal Antibodies & T Cell Products PDF by David H Katz Full Free and published by CRC Press. This book was released on 2019-06-04 with total page 187 pages. Available in PDF, EPUB and Kindle. Book excerpt: First Published in 1982, this book offers a full, comprehensive guide into the applications of Monoclonal Antibodies. Carefully compiled and filled with a vast repertoire of notes, diagrams, and references this book serves as a useful reference for Students of Medicine, and other practitioners in their respective fields.


Download Monoclonal Antibodies in Biotechnology PDF

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Publisher : Cambridge University Press
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ISBN 10 : 9780521258906
Pages : 401 pages
Rating : 4.5/5 (521 users)

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Download Monoclonal Antibodies in Biotechnology PDF by Kenneth C. McCullough Full Free and published by Cambridge University Press. This book was released on 1990-04-19 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive description of principles, methodologies and applications of Monoclonal antibodies to modern science and industry.


Download Monoclonal Antibodies PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9781461575054
Pages : 424 pages
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Download Monoclonal Antibodies PDF by Roger H. Kennett Full Free and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: On August 7, 1975, Kohler and Milstein published in Nature (256:495) a report describing "Continuous cultures of fused cells secreting antibody of predefined specificity. " Their report has become a classic and has already had a profound effect on basic and applied research in biology and medicine. By the time the first Workshop on Lymphocyte Hybridomas (Current Topics in Microbiology and Im munology 81, 1978) was held on April 3-5, 1978, in Bethesda, Maryland, investi gators from many laboratories had made hybrids between plasmacytomas and spleen cells from immunized animals and had obtained monoclonal antibodies reacting with a broad variety of antigenic determinants. At the time Kohler and Milstein introduced this new technology, the editors of this volume were involved in the production of antisera against differentiation antigens (K. B. B. ), histocompatibility antigens (T. ]. McK. ), and human tumor associated antigens (R. H. K. ). Because of the potential usefulness of monoclonal antibodies in these areas, we each began production of hybridomas and analysis of the resulting monoclonal reagents. One of the most interesting aspects of participation in the early stages of the development and application of hybrid oma technology has been observing how the implications of the initial observa tions gradually spread first among the practitioners of immunology and immu nogenetics, and then to other areas of the biological sciences, such as developmental biology, biochemistry, human genetics, and cell and tumor biology.


Download Monoclonal Hybridoma Antibodies PDF

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Publisher : CRC Press
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ISBN 10 : 9781351091725
Pages : 252 pages
Rating : 4.3/5 (351 users)

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Download Monoclonal Hybridoma Antibodies PDF by John G.R. Hurrell Full Free and published by CRC Press. This book was released on 2018-05-04 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume.


Download Human Hybridomas and Monoclonal Antibodies PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9781468449495
Pages : 526 pages
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Download Human Hybridomas and Monoclonal Antibodies PDF by Edgar Engleman Full Free and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 526 pages. Available in PDF, EPUB and Kindle. Book excerpt: Soon after Kohler and Milstein described the use of somatic cell hybridization for the production of murine monoclonal antibodies of desired specificity, this relatively simple technique became widely applied. Indeed, production of murine monoclonal antibodies is now considered routine by immunologists and nonimmunologists alike. However, as heterologous proteins, mouse monoclonal antibodies have one major limitation: they are immunogenic in man and, hence, their use in vivo is severely limited. An obvious solution to this problem is to produce human hybridomas with the same techniques used for the production of rodent hybrids. Unfortunately, the history of human hybridomas has been marked by substantive and often exasperating tech nical problems, and the first reports of hybrids secreting human immu noglobulin of desired specificity did not appear until 1980. These reports were met with initial enthusiasm, but it soon became apparent that while human lymphocytes might be fused, their frequency, level of Ig synthesis, and stability were such that production of human antibodies with this method was neither routine nor practical. Nonetheless, a sufficient number of investiga tors persevered, and during the next 5 years relatively efficient B-cell fusion partners as well as improved methods of Epstein-Barr virus transformation were developed. Generation of human T -T hybrids has also been achieved, although problems of chromosomal stability remain a substantial obstacle, more so than with B-cell lines.


Download Neuroblastoma PDF

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Publisher : CRC Press
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ISBN 10 : 0849301572
Pages : 430 pages
Rating : 4.3/5 (31 users)

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Download Neuroblastoma PDF by Carl E. Pochedly Full Free and published by CRC Press. This book was released on 1990-06-21 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this book is to provide a comprehensive review of current knowledge and to give a thoughtful assessment of the many complex issues involved in the diagnosis and treatment of this common malignant solid tumor found in children. This up-to-date publication also reviews new concepts in histogenesis and histopathology of neuroblastoma. Divided into three main sections, this work focuses on tumor biology, clinical management, and prognosis and future perspectives. This fascinating work includes in-depth discussions on neuroblastoma in infancy and the manifestations of this tumor as it affects various organs and various parts of the body. It also provides guidelines for treatment with surgery, radiotherapy and chemotherapy. This ideal resource is loaded with information for all those who care for children with cancer, including pediatric oncologists, medical oncologists, pathologists, radiotherapists, pediatric nurse oncologists and pediatricians.


Download Current Catalog PDF

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ISBN 10 : STANFORD:36105009872693
Pages : pages
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Download Current Catalog PDF by National Library of Medicine (U.S.) Full Free and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:


Download Expression of Differentiated Functions in Cancer Cells PDF

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ISBN 10 : WISC:89009173311
Pages : 552 pages
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Download Expression of Differentiated Functions in Cancer Cells PDF by Roberto P. Revoltella Full Free and published by . This book was released on 1982 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt:


Download Disease Markers PDF

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ISBN 10 : NWU:35558000389797
Pages : pages
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Download Disease Markers PDF by Full Free and published by . This book was released on 1983 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:


Download Monoclonal Hybridoma Antibodies Techqs & Applns PDF

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Publisher : CRC PressI Llc
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ISBN 10 : UOM:39015000319031
Pages : 248 pages
Rating : 4.3/5 (39 users)

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Download Monoclonal Hybridoma Antibodies Techqs & Applns PDF by John G. R. Hurrell Full Free and published by CRC PressI Llc. This book was released on 1982-07 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first section of this volume is aimed to provide a comprehensive review of the many varied and often empirically derived techniques and procedures currently in use to produce monoclonal hybridoma cell lines and to characterize the antibodies secreted. The goal has been achieved with the chapter contributed by Zola and Brookes who, as each step in the process of hybridoma production and antibody characterisation is reviewed, have provided an experimental procedure found to be satisfactory in their laboratory.The second section of this volume is designed to provide a review of areas in which monoclonal hybridoma antibodies have been of particular advantage. This is a rapidly advancing field which could not be thoroughly reviewed in a single volume.


Download Cell Culture in the Neurosciences PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9781461324737
Pages : 383 pages
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Download Cell Culture in the Neurosciences PDF by Jane Bottenstein Full Free and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: A fundamental problem in neuroscience is the elucidation of the cellular and molecular mechanisms underlying the development and function of the nervous system. The complexity of organization, the heteroge neity of cell types and their interactions, and the difficulty of controlling experimental variables in intact organisms make this a formidable task. Because of the ability that it affords to analyze smaller components of the nervous system (even single cells in some cases) and to better control experimental variables, cell culture has become an increasingly valuable tool for neuroscientists. Many aspects of neural development, such as proliferation, differentiation, synaptogenesis, and myelination, occur in culture with time courses remarkably similar to those in vivo. Thus, in vitro methods often provide excellent model systems for investigating neurobiological questions. Ross Harrison described the first culture of neural tissue in 1907 and used morphological methods to analyze the cultures. Since that time the technique has been progressively modified and used to address an ever widening range of developmental questions. In recent years a con vergence of new or improved cell culture, biochemical, electrophysiol ogical, and immunological methods has occurred and been brought to bear on neurobiological questions. This volume is intended not to be comprehensive but rather to highlight some of the latest findings, with a review of previous important work as well, in which combinations of these methods are used.


Download Ganglioside Structure, Function, and Biomedical Potential PDF

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Publisher : Springer Science & Business Media
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ISBN 10 : 9781468412000
Pages : 649 pages
Rating : 4.4/5 (468 users)

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Download Ganglioside Structure, Function, and Biomedical Potential PDF by Robert W. Ledeen Full Free and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 649 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the proceedings of the symposium on "Ganglioside Structure, Function and Biomedical Potential" which was held at Parksville, Vancouver Island, B. C. , Canada on July 6-10, 1983. The symposium was organized ao a satellite to the ninth meeting of the International Society for Neurochemistry, held immediately afterward in Vancouver City, B. C. Close to 50 speakers from 9 countries presented papers on a wide range of topics on the ganglioside theme. These encompassed the many aspects of basic research that have evolved over the past half-century, as well as some newer topics relating to the biomedical potential of gangliosides as therapeutic agents. One of the purposes of the meeting was to encourage dialogue between investigators in these seemingly diverse areas in the hope that each would come away with fresh perspective toward his own work. Judging from the many spirited, informed discussions that took place throughout the four day meeting, this goal was achieved. The charm and beauty of Vancouver Island undoubtedly contributed to the congenial atmosphere which quickly developed among the 120 or so participants. Drawn from 13 countries of Europe (East and West), America (North and South), Asia and the Middle East, this gathering reflected the broad geographical scale on which ganglioside research is now conducted. That this field is still in its "logarithmic" stage of growth is indicated by the increasing number of citations appearing each year in the various abstracts (e. g. fig. next page).


Download Structure and Function of Antibodies PDF

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Publisher : MDPI
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ISBN 10 : 9783039438976
Pages : 440 pages
Rating : 4.0/5 (39 users)

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Download Structure and Function of Antibodies PDF by Roy Jefferis Full Free and published by MDPI. This book was released on 2021-02-05 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a detailed description of all kinds of therapeutic antibodies including IgGs, IgAs, IgEs, and IgMs, bispecific antibodies, chimeric antigen receptor antibodies, and antibody fragments. Details about how each of these antibodies interact with their ligands, the immune system, and their targets are provided. Additionally, this book delves into the details of antibody, Fc, and variable chain structures, and how subtle changes in structure, charge, flexibility, post-translational modification, and the ability to bind to natural antibody ligands can result in a significant impact on antibody activity and functionality. Finally, the book explains the critical quality attributes of modern therapeutic antibodies and how to ensure that antibodies entering development have the best possible chance of success.